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Abstract Dinitrogen (N₂) fixation supports marine life through the supply of reactive nitrogen. Recent studies suggest that particle-associated non-cyanobacterial diazotrophs (NCDs) could contribute significantly to N₂fixation contrary to the paradigm of diazotrophy as primarily driven by cyanobacterial genera. We examine the community composition of NCDs associated with suspended, slow, and fast-sinking particles in the North Pacific Subtropical Gyre. Suspended and slow-sinking particles showed a higher abundance of cyanobacterial diazotrophs than fast-sinking particles, while fast-sinking particles showed a higher diversity of NCDs includingMarinobacter,OceanobacterandPseudomonas. Using single-cell mass spectrometry we find that Gammaproteobacteria N₂fixation rates were higher on suspended and slow-sinking particles (up to 67 ± 48.54 fmol N cell⁻¹ d⁻¹), while putative NCDs’ rates were highest on fast-sinking particles (121 ± 22.02 fmol N cell⁻¹ d⁻¹). These rates are comparable to previous diazotrophic cyanobacteria observations, suggesting that particle-associated NCDs may be important contributors to pelagic N₂fixation. 

Industrial activities have increased the supply of iron to the ocean, but the magnitude of anthropogenic input and its ecological consequences are not well-constrained by observations. Across four expeditions to the North Pacific transition zone, we document a repeated supply of isotopically light iron from an atmospheric source in spring, reflecting an estimated 39 ± 9 % anthropogenic contribution to the surface ocean iron budget. Expression of iron-stress genes in metatranscriptomes, and evidence for colimitation of ecosystem productivity by iron and nitrogen, indicates that enhanced iron supply should spur spring phytoplankton blooms, accelerating the seasonal drawdown of nitrate delivered by winter mixing. This effect is consistent with regional trends in satellite ocean color, which show a shorter, more intense spring bloom period, followed by an earlier arrival of oligotrophic conditions in summer. Continued iron emissions may contribute to poleward shifts in transitional marine ecosystems, compounding the anticipated impacts from ocean warming and stratification. 

The proportions of carbon (C), nitrogen (N), and phosphorus (P) in surface ocean particulate matter deviate greatly from the canonical Redfield Ratio (C:N:P = 106:16:1) in space and time with significant implications for global carbon storage as this matter reaches the deep ocean. Recent work has revealed clear latitudinal patterns in C:N:P, yet the relative importance of ecological, physiological, or biochemical processes in creating these patterns is unclear. We present high-resolution, concurrent measurements of particulate C:N:P, macromolecular composition, environmental conditions, and plankton community composition from a transect spanning a subtropical-subpolar boundary, the North Pacific Transition Zone. We find that the summed contribution of macromolecules to particulate C, N, and P is consistent with, and provides interpretation for, particulate C:N:P patterns. A decline in particulate C:N from the subtropical to subpolar North Pacific largely reflects an increase in the relative contribution of protein compared to carbohydrate and lipid, whereas variation in C:P and N:P correspond to shifts in protein relative to polyphosphate, DNA, and RNA. Possible causes for the corresponding trends in C:N and macromolecular composition include physiological responses and changes in community structure of phytoplankton, which represented approximately 1/3^rdof particulate C across the transect. Comparison with culture experiments and an allocation-based model of phytoplankton macromolecular composition suggest that physiological acclimation to changing nutrient supply is the most likely explanation for the latitudinal trend in C:N, offering both a mechanistic interpretation and biochemical basis for large-scale patterns in C:N:P. 

Abstract In vitro incubations using natural marine communities can provide insight into community structure and function in ways that are challenging through field observations alone. We have designed a minimal metal incubation system for controlled and repeatable experimentation of microbial communities. The systems, dubbed Pelagic Ecosystem Research Incubators (PERIcosms), are 115 L, conical tanks designed to sample suspended, settled, and wall associated material for month long periods. PERIcosms combine some of the ecological advantages of large volume mesocosm incubations with the experimental ease and replication of bottle incubations, and their design is accessible for use by researchers without specialized training or travel to a designated incubation facility. Here, we provide a detailed description for the construction and implementation of PERIcosms and demonstrate their potential to promote replicable, diverse communities for several weeks under clean conditions using time‐series results from two field experiments. One field experiment utilized coastal waters collected from Santa Catalina Island, CA and the other oligotrophic waters collected offshore of Honolulu, HI. Biomass metrics (chlorophyll a and particulate carbon) along with 16S/18S DNA based community composition assessments were conducted to show that communities contained within PERIcosms remained alive and diverse for several weeks using a semi‐continuous culturing approach. We detail trace metal clean techniques that can be used to minimize external contamination, particularly for low dissolved iron environments. PERIcosms have the potential to facilitate natural community incubations which are needed to continue advancing our understanding of microbial ecology and geochemistry. 

Abstract The combination of taxa and size classes of phytoplankton that coexist at any location affects the structure of the marine food web and the magnitude of carbon fluxes to the deep ocean. But what controls the patterns of this community structure across environmental gradients remains unclear. Here, we focus on the North East Pacific Transition Zone, a ~ 10° region of latitude straddling warm, nutrient‐poor subtropical and cold, nutrient‐rich subpolar gyres. Data from three cruises to the region revealed intricate patterns of phytoplankton community structure: poleward increases in the number of cell size classes; increasing biomass of picoeukaryotes and diatoms; decreases in diazotrophs andProchlorococcus; and both increases and decreases inSynechococcus. These patterns can only be partially explained by existing theories. Using data, theory, and numerical simulations, we show that the patterns of plankton distributions across the transition zone are the result of gradients in nutrient supply rates, which control a range of complex biotic interactions. We examine how interactions such as size‐specific grazing, multiple trophic strategies, shared grazing between several phytoplankton size classes and heterotrophic bacteria, and competition for multiple resources can individually explain aspects of the observed community structure. However, it is the combination of all these interactions together that is needed to explain the bulk compositional patterns in phytoplankton across the North East Pacific Transition Zone. The synthesis of multiple mechanisms is essential for us to begin to understand the shaping of community structure over large environmental gradients.